![](https://parts.igem.org/images/partbypart/icon_intermediate.png)
Intermediate
Part:BBa_K1521002:Design
Designed by: Danilo Keiji Zampronio Group: iGEM14_USP-UNESP-UFSCar-BRA (2014-10-17)
LasR functional construction + GFP reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2174
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2055
Illegal BglII site found at 4576 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 585
Illegal AgeI site found at 782
Illegal AgeI site found at 1617 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2946
Design Notes
Note that for the proper activity of LasR we added the BBa_K081001, which codify for LasI responsable for the homoserine lactone (HSL) production.